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This novel protein is the fourth most frequently-detected in latent BC-1 based on total peptide spectra corresponding to one ORF (3 total).
The light, intermediate and heavy dimethyl labeled samples were mixed in 1∶1∶1 ratio based on total peptide amount, determined by analyzing an aliquot of the labeled samples on a regular LC-MS run and comparing overall peptide signal intensities.
The peptides were labeled with isobaric tags and mixed at 1 1 ratio based on total peptide amount.
After SDS-PAGE, analysis of the 43 kDa band by mass spectrometry identified the dominant protein (based on total peptide counts) as TbGSK3short (Tb927.10.13780), a CDXG kinase previously found to be essential in BSF T. brucei (Ojo et al., 2008).
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Imaging functionals based on total variation and l-1 minimization.
Compensation rank is based on total compensation for latest fiscal year.
*Percentages based on total state population.
In total, these combined 366 protein identifications were based on 1089 unique peptide hits from a total of 3051 peptide hits.
Based on the total number of peptides identified, the majority of components found in the C. gestroi digestome were cellulose-degrading enzymes.
The proportion assigned to each category is based on the total number of peptides of all the proteins identified and contributing to that category; although this method is not completely rigorous, it does permit comparison of the two samples.
Based on the total number of peptides identified, the majority of components found in the C. gestroi digestome were cellulose degrading enzymes, including β-glucosidases (GH1 and GH3), which accounted for up to 10% of the protein matches, followed by GH5, GH7 and GH9 families, which made up 22% of the total peptides.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com