Maximum PHA concentration of 19.7 g/l, product yield coefficient (based on total carbohydrates) of 0.87 and 0.21 g/l h volumetric productivity were achieved.
On the other hand, the ethanol yield, of about 31% of the maximum theoretical based on total carbohydrates content, achieved in the present work indicates that further investigation of the process is required.
The test rices contained 50 g of glycaemic (available) carbohydrate, based on total starch of the cooked rice, and the reference food (glucose drink) had been tested in each volunteer on three previous occasions.
Hydrolysis constants of carbohydrates and proteins were estimated based on total short chain fatty acids (SCFA) and ammonia production in vitro.
*Percentages based on total state population.
To better characterize the Acai polysaccharide agonist, the Acai-PS extract was then fractionated by preparative Sepharose 6B size-exclusion chromatography to obtain three fractions, which were selected based on the total carbohydrate elution profile (designated as: Acai-1, Acai-2, and Acai-3, Figure 3A).
The acidic and neutral Diaion-unbound fractions (CSP-AU and CSP-NU, respectively) were subsequently fractionated by preparative Sepharose 6B size-exclusion chromatography to obtain four sub-fractions, which were selected based on the total carbohydrate and polyphenol elution profiles (designated as CSP-AU1, CSP-AU2, CSP-NU1, and CSP-NU2) (Table 2).
Moreover, dietary interventions using high total fat based on low-carbohydrate, high-MUFA and PUFA strategies (Mediterranean diet) have been shown to reduce cardiovascular events without weight loss or exercise.
a sugar yield based on the carbohydrate in the raw material; b glucan digestibility based on the carbohydrate in the pretreated solid.
