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The pathological classification was determined based on tissue sections with haematoxylin-eosin staining and immunohistochemistry as well as clinical characteristics.
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However, few if any molecular studies of the cerebral vasculature in schizophrenia have been attempted, and existing quantitative molecular studies based on tissue homogenate or sections are unlikely to include a signal from the relevant cells as vasculature accounts for only 0.1% of whole brain tissue [20].
Simulations were based on tissue characteristics observed in pathology slides.
Limitation based on tissue rarity.
Limitation based on tissue consent.
The mean squared rank was chosen based on a survey of statistical approaches for gene set analysis [ 34], and out-performed other summary statistics in a series of classification benchmarks based on tissue-specific pathway expression (see benchmarking section below).
Based on our observations on tissue sections we believe that 1% acid fast stain, Gram's chromotrope stain and GMS stain have several advantages over other stains and therefore suggest the use of these in providing a reliable diagnosis of microsporidia.
SKS performed the pathology on tissue sections.
In situ hybridization on tissue sections was performed on 10-μm sections from paraffin-embedded tissue.
Immunohistochemistry on tissue sections was performed as described previously [ 8].
In addition, absorption tests were performed on tissue sections.
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