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Previous studies based on tissue microarray data indicated that DEK mRNA is overexpressed in bladder tumors [ 17].
Based on tissue microarray analyses, we observed a significant downregulation of SLC22A1 protein expression in HCC compared to normal adjacent tissue (P < 0.0001).
Clinically, using RF, tumour class discovery of renal cell carcinoma based on tissue microarray profiling is possible, and this could not be explained by clinicopathological variables (Shi et al, 2005).
Figure 2 presents retrospective mRNA expression based on tissue microarray (TMA) analysis of LOX and LOXL1 LOXL4 in breast cancer tissue biopsy samples from 176 patients with breast cancer versus 10 healthy control human breast tissue samples.
Previously conducted studies have been based on tissue microarray analysis (Al-Mulla et al, 2006; Minoo et al, 2007; Zlobec et al, 2008; Doyle et al, 2013), which may cause considerable underestimation of the true distribution of RKIP expression based on the well-described genetic heterogeneity of CRC (Gerlinger et al, 2012).
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However, most of these results are based on tissue microarrays.
IHC was performed on either whole sections, or for the retrospective, population based series, on tissue microarray slides in which duplicate 0.6 mm cores from each case were present in the array.
For the microarray experiments, RNA was pooled in an equal molar ratio from the three biological replicates based on tissue and genotype.
Limitation based on tissue rarity.
Limitation based on tissue consent.
This situation along with conflicting interpretations from sporadic clinical investigations in this field prompted us to perform a comprehensive study on the issue based on a tissue microarray covering >400 individual CRC tumour samples from a particularly homogeneous patient cohort.
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