Sentence examples for based on threshold cycle from inspiring English sources

All comparisons were based on threshold cycle number itself, rather than transformed estimates of RNA quantity, because the former value best reflects experimental error.

Amount of DNA immunoprecipitated with FLAG and IgG beads was calculated based on threshold cycle [C t)] using the ΔC t) method and normalized to input samples.

The results derived from qRT-PCR were analyzed based on threshold cycle (Ct) values.

Since the careful data analysis is exclusively based on threshold cycle (CT) values and the shift in CT values, a direct comparison with the correlation coefficients obtained by us using a different methodology is not possible.

Standard curves were run with 50, 10, 2 and 0.4 ng of pooled cDNA from all samples, and relative expression was then determined from the standard curves based on threshold cycle (Cq) value and absolute efficiency values using the ΔCq method implemented in the Bio-Rad CFX Manager v2.0 software.

The standards were used to calculate a regression of threshold cycle on molecule copy number to determine a log value of starting abundance for each of the cDNA samples based on their threshold cycle.

Relative expression levels of genes were calculated based on the threshold cycle (CT) deviation of the treated sample versus a control and expressed in comparison to reference gene.

Quantitative PCR values were calculated based on the threshold cycle (Ct) and were normalized to input controls.

The relative mRNA amount of each sample was calculated based on its threshold cycle, Ct, in comparison to the Ct of housekeeping gene β-actin.

Quantitation of the PCR results were calculated based on the threshold cycle (Ct) and were normalized using β-tubulin for all mouse transcripts or TATA Binding Protein TBPP) for all human transcripts.

The expression of miRNA was defined based on the threshold cycle (Ct), and relative expression levels were calculated as 2-[(Ct of miR-96) – (Ct of U6)] after normalization with reference to expression of U6 small nuclear RNA.