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Statistics analysis was based on three technical replicates.
Spread of standard deviation methylation values across Watson strand amplicons, based on three technical replicates.
Relative quantitation was carried out using ΔΔCT method based on three technical replicates.
Standard deviation (SD) calculations are based on three technical replicates of two independent biological repeats.
Means of Ct values are based on three technical replicates each of two biological replicates.
Transcript levels were quantified in three biological replicates, each based on three technical replicates.
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Based on two "Technical Reports ", archaeologist Sabino Quispe Serrano, attach�� to the Dirección de Coordinación de Calificación de Intervenciopnes Arqueológicas, declared the research project presented by Thierry Jamin and the Inkari Institute as "unfair".
This assay is based on two technical advancements.
The mean Cp value was calculated for each cDNA sample based on five technical replicates.
RT-qPCR was carried out as described previously (Kosawang et al. 2014), except that each treatment was measured in a minimum of three biological replicates, each based on two technical replicates.
Expression shown relative to the expression in naive eggs, the mean fold change of the biological replicates (based on two technical replicates) is plotted and error bars show the standard error.
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