Sentence examples for based on these nucleotide from inspiring English sources
Based on these nucleotide sequences, two PCR primers were designed to amplify the region around the SalI site in the UL17 gene and the PCR was carried out using 78 field isolates from various parts of Japan.
Based on these nucleotide differences, specific primers were designed to perform expression analysis; the results showed that it expressed in both fruit and leaf, its expression level was obviously lower than that of FPPS1 gene in fruit which was stored at 4 °C for 5 weeks.
Based on these alignments, nucleotide alignments were performed manually, and phylogenetic relationships were inferred by maximum likelihood (ML).
The example just showed that we could now use the database to prioritize our efforts and guide further studies based on these specific nucleotides.
Degenerate primers P1, P2, P5, and P6 (Additional file 4: Table S2) were designed based on the nucleotide sequences in these regions and used at 0.2 μM in PCR reactions containing 1.6 μl cDNA preparation, 0.1 mM of each dNTP, 2 mM MgCl2, and 0.5 μl peqGOLD Taq DNA polymerase (PEQLAB) in a total volume of 50 μl peqGOLD Taq buffer.
Based on the nucleotide sequences of these unique sequence clusters, 171 'unique deduced protein fragments' (UPFs) were predicted (average 50 UPFs per plant) that represented the predicted amino acid variants of the first variable domain of alpha-gliadins that are expressed in the endosperm.
The boundaries of these clusters were based on the nucleotide positions of the first and the last mutations in that particular group.
Based on the nucleotide polymorphisms observed between each Hd6 region, we examined the phylogenetic relationships among the varieties and species.
Based on these results, we adopted modified nucleotide SELEX exclusively in our standard selections.
