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Based on these constructs, a series of substrate and MgZ variants were synthesized and tested to assess the catalytic importance of P1, P2 and P3.
In order to predict the behavior of these constructs, modeling and simulation of their biomolecular reaction networks are needed to enable the design and fabrication of both the constructs themselves and physical devices based on these constructs.
Mouse Foxo1 plasmid and its mutants were generously given by Dr A Miyajima (The University of Tokyo, Japan) and GAL4-DBD-FOXO1 and its mutants were cloned based on these constructs.
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The approach relies on analyzing transient system responses (local dynamics) in the frequency energy domain, and based on these, constructing damped frequency energy plots – FEPs (global dynamics) under the assumption of weak damping.
Based on these criteria, all constructs proposed were characterized by adequate reliability (Table 6).
Initial judgments based on these pre-existing constructs in relation to the context of the new case, lead to the construction of a frame 17 that limits and gives direction to the rest of the cognitive process.
Based on these hypotheses we construct possible scenarios.
Based on these finding, we constructed scrB/OP-ΔfruA and scrB/OP-ΔfruK strains, which showed significant increase in HA productivity (Fig. 6b).
Based on these genes/miRNAs, we constructed conditional combinatorial gene regulatory networks (cGRNs) involving both transcription factors (TFs) and miRNAs.
However, design based on these methods requires constructing separate S-N curves from the fatigue testing based on the joint geometry, loading, thickness, etc.
Based on these findings, we constructed an engineered yeast strain for the improved identification of signal promotion by Gαi-specific human GPCRs using flow cytometry.
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