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After constructing the homology graph based on these alignments, one postspeciation duplication was inferred.
All Brassica napus gene sequences matching a particular Arabidopsis candidate gene were aligned and based on these alignments gene-specific oligonucleotide pairs were developed.
Based on these alignments, we identified 14,751 penguin-specific amino acid changes (one genotype in both of the two penguins and another genotype in their close relatives) in 4922 genes, including deletions and insertions (Additional file 13: Table S11).
Based on these alignments phylogenetic trees were inferred using Bayesian Inference and Maximum Likelihood methods.
Based on these alignments HMMs were created and calibrated by hmmbuild and hmmcalibrate HMMER-package [39].
Based on these alignments, any regions without significant similarity (E≤1e-6) were cropped, leaving only the SSU rRNA gene.
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Thus, identification of gene structures conflicting with these spliced alignments was performed automatically, but updates to individual genes based on these spliced alignments were carried out manually using Neomorphic's Annotation Station (Neomorphic was acquired by Affymetrix on 10/31/2000).
We systematically removed the fastest evolving sites one at a time, and rebuilt ML phylogenies based on these reduced alignments.
Based on these BES alignments and the build of contig 20, SC4 and SC13 were merged together forming a new SC of over 30 Mb, thus extending the draft sequence assembly.
Finally, based on these orthologous alignments for each group, we constructed five amino acid exchange matrices.
I-value points to an optima for the sieved Alignment 1 at [NEquiv,RMSD] of [101,3.7 Å], whereas DALI points at [94, 3.17 Å]. (Superpositions based on these two alignments can be found in the online supplementary material).
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CEO of Professional Science Editing for Scientists @ prosciediting.com