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The phylogenetic associations of cooS fragments were predicted by using a combination of a sliding window approach to reconstruct phylogenetic trees based on their translated amino acid sequences (which are not included in the construction of trees shown in figures) and by comparing their indels (as described above) to those found in full-length or near-full-length CooS sequences.
In brief, we aligned the nucleotide sequences based on their translated amino acid sequences, and the aligned sets of genes were concatenated for use in subsequent analyses.
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Scaffolds generated by SPROUT were evaluated based on their ability to be translated into structures that were synthetically tractable.
Finally, 73 out of 231 genes were found to be potentially translated based on their signal in polysome-associated RNA-Seq representing transcripts that are actively being translated.
Viral genes are translated based on their requirements during different stages of the viral life cycle.
Protein sequences encoded by identified orthologous gene pairs were aligned with ClustalW [ 43], and then back-translated into nucleotide sequences based on their original sequences.
The MADL framework is capable of generating two types of cycle accurate simulators, one based on the dynamic-operation state machine (OSM) model in MADL, and the other an optimized one based on the translated static-OSM model.
The international consistency of the coding was evaluated based on two translated interviews (one patient interview and one GP interview) that were sent to Richard Baker.
Since the original Los Alamos MSA does not always maintain the reading frame, codon-based MSAs were created for each gene based on the translated protein MSAs created using the MUSCLE program [ 40].
Some of the larger American publishers said monolingual editors fear making risky buying decisions based on short translated excerpts.
Phylogenetic analyses based on the translated sequences reiterate this relationship between R. flavefaciens and the Clostridia.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com