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The relative abundance of the 25 most abundant proteins identified is provided in Additional file 4. GO-based annotation was used to categorize the proteins based on their subcellular localization, molecular function and biological processes.
Importantly, these methods allow us to detect potential posttranslational modification of the mKIAA/KIAA proteins and to predict their biological function based on their subcellular localization.
Based on their subcellular localization, the PRXs in Arabidopsis comprise four subgroups: four PRXs (2-CysPrxA, 2-CysPrxB, PrxQ, and PrxIIE), localized in the chloroplast; a PrxIIF, in the mitochondrion; a 1-CysPrx, in the nucleo-cytoplasm; and three (PrxIIB, PrxIIC, and PrxIID), in the cytosol (Dietz 2011).
In this article, we address the problem of classifying proteins based on their subcellular localization patterns.
All the network's proteins were categorized based on their subcellular location.
The identified proteins further were classified based on their subcellular localization and biological process according to annotations in the Swiss-Prot database.
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Typical DUSPs can be further divided into three groups based on their predominant subcellular locations (nuclear, cytoplasmic, or dually-located), with this grouping also coinciding with their sequence alignment.
Gaudí LxBBW is the best option when fixation and immunostaining are required, as a single α-GFP antibody can be used to recognize three FP outputs based on their differential subcellular localization (Fig. 2C,D).
Based on their predicted subcellular localization, it is most likely that tRNase ZS2 and tRNase ZL1 are involved in the 3'-ends processing of pre-tRNAs in the chloroplasts, and both the nucleus and mitochondria, respectively, whereas tRNase ZL2 either serves as a back-up for mitochondrial pre-tRNA 3'-end processing or plays a specialized, yet to be discovered function.
HDACs comprise a large group of proteins divided into four classes based on their homologies to yeast HDACs, their subcellular localizations and their enzymatic activities [8], [9].
In mammals carbonic anhydrase has sixteen different isozymes based on their distribution in tissues and subcellular localization.
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