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These sequences were annotated based on their overlap with publicly available genome annotations (Table S1) and a total of 472 known miRNAs were identified with a greater total number of miRNAs expressed in the normal donor group than in the patient group (Fig. 2A).
This is achieved through a 'landscape-species approach' (sensu [3]), which maps spatially-explicit ecological requirements for a suite of conservation targets ('landscape species') and, based on their overlap with human land uses, identifies key threats to be addressed by conservation action [3], [34], [35].
Resulting intersection file validates a minimum of 147 TSS sites based on their overlap.
Probes/CpGs were annotated based on their overlap with specific genomic features using BedTools version 2.1 [ 57].
The variants were further flagged as novel or present in either dbSNP138 or COSMIC (v67) databases, based on their overlap.
The CRMs or single TFs were then broken down into categories based on their overlap with ChIP-seq data in macaque, dog, mouse, and rat.
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Cis-NAT pairs can be classified into three groups based on their overlapping patterns, including 3' end overlap, 5' end overlap and one entirely contained within the other [ 49].
Therefore, we think that it is more reasonable to apply the method (A, B, and C) to each cohort and then reconcile the candidate genes from the three cohorts to reach a final list of prognostic genes based on their overlaps among any two cohorts and concordant expression across the three cohorts.
The classification of cis-NAT pairs based on their overlapping patterns was shown in Table 4.
The large number of short sequences produced, termed reads, are assembled into longer sequences (contigs) based on their overlaps, potentially providing the DNA sequence of an entire genome.
3) WGS: Each individual eBAC was treated as a mini-genome and the standard ATLAS-WGS assembly procedure was applied, including detecting overlaps among the reads, filtering conflicting overlaps based on overlap patterns, clustering reads into bins based on their overlaps and PHRAP assembly in each bin.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com