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The software allows excluding and assigning putative fathers based on their multi locus genotypes.
Among the list of 376 genotypes obtained, the distinct genets were recognized based on their multi locus genotypes (MLGs) assessed with the 9 microsatellite markers following [ 58].
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Individuals are then assigned probabilistically to one or more clusters based on their multi-locus genotype.
Captive individuals were assigned to island, species and, in many cases, population based on their multi-locus genotypes using two different approaches.
Individuals of unknown ancestry were assigned to island populations based on their multi-locus genotypes using two separate approaches.
Clustering of individuals based on their multi-locus genotypes revealed substantial hierarchical structure among populations across the species range (Fig. 3).
Population likelihood assignment tests for individuals based on their multi-locus genotype were used to estimate contemporary, effective seed dispersal events.
These tests can simultaneously define naturally occurring clusters of individuals based on their multi-locus genotypes and assign individuals to clusters.
To help bias marker selection in favor of such variants, we devised a process that prioritizes annotated SNPs for genotyping studies based on their location within Multi-species Conserved Sequences (MCSs) and used this process to select SNPs in a region of linkage to a complex disease.
Markers were chosen based on their location in the map, multi-allelism and minimum of 50% heterozygosity.
After adsorption equilibrium was achieved (approximately 30 minutes), followed by centrifugation, the free protein molar concentrations of GC3 and CC17 were measured based on their fluorescence readings by the BioTek multi-detection microplate reader (Winooski, VT, USA).
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