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For unbiased identification of the PWMs that share sequence similarity with the STAT3-specific PWM, V$STAT3_01, a total of 565 PWMs derived from vertebrate TRANSFAC database [32] were clustered based on their motif similarity (Figure S1).
Based on their motif data, we identify 539 significant motifs from the promoter regions after removing the redundancy.
To this end, we first determine the similarity (Pearson correlation coefficient) between each pair of genes based on their motif scores.
To our knowledge, there is no obvious comparator in the literature for Regulus as a similarity score of two CRMs based on their motif compositions.
Again, when these peptides were further sub-divided into those predicted to bind to HLA-A*68 02 HLA-A*68 023 bandd on their motif, an increased repertoire was observed for both allomorphs in the absence of TAPBPR.
A total of 2,903 SSRs were identified and categorized into five groups based on their motif type: (AC n, (AG n, (AT n, (GC n, and T/T ("T/T" for tri- and tetra-nucleotides).
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In earlier studies, PDZ domains were grouped into different specificity classes based on their target motif structures: X[T/S]Xφ-COOH (Class I motif), XφXφ-COOH (Class II motif) and a minor X[D/E]Xφ-COOH (Class III motif), where X represents any natural amino acid and φ represents hydrophobic amino acid [ 9, 12].
To improve alignments, the 376 sequences were split into three groups based on their signature motif alignment pattern.
These Kunitz peptides were categorized in three different groups (groups I, II and III) based on their Cys motif.
All peptides were divided into groups based on their Cys motif (i.e., the number intra-Cys residues) and a group was defined as whether there were two or more representative sequences for each motif.
Organizing the I. ricinus single Kunitz-domain peptides based on their cysteine motif allowed us to specify a putative target and to relate this target specificity to Illumina transcript reads during tick feeding.
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