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To compare the expression levels across the libraries, we normalized the frequency of signatures in each library to one million (transcripts per million or TPM). Figure 1 shows the number of pooled distinct signatures from the four MPSS libraries separated by reliability and significance filters and further grouped based on their match in the Nipponbare genomic sequence.
Targets are identified based on their match to positions 2-8 of the mature miRNA (m8: the seed + position 8).
It is worth emphasizing that all 126 natural ligands had been selected based on their match with the last four C-terminal amino acids of artificial ligands only.
We ranked all putative interaction partners based on their match scores and subsequently analyzed, through Area under the ROC Curve (AuC) analysis, whether the identified interaction partners of dysferlin were among the top ranked proteins.
This enables us to only recall suitable codes for each value and sort them based on their match.
We next classified reads based on their match to genomic features into eight pools of small RNAs, namely rRNA, tRNA, small nucleolar RNAs, milRNA (Lee et al. 2010b), Dicer-independent small interfering RNA (Lee et al. 2010b), masiRNA, mitochondrial RNA, and other unspecified RNAs.
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The annotated Unigenes were functionally classified based on their matches in the GO, COG and KEGG databases.
By contrast, 21 of the additional clusters were of transcripts derived from duplicated genes or from conserved gene families, based on their matches to different locations in the draft genome sequence.
Additionally, whole-genome assembly can be significantly enhanced using an integrated map to orient and order sequence scaffolds along the length of linkage groups based on their matches with BES, allowing generation of chromosome-scale scaffolds.
Eleven were composed of 2 assembled sequences that are likely allelic variants of the same genes, based on their matches to a single location in a preliminary draft assembly of the Daphnia genome sequence at the mid-point of the genome sequencing project (4 × coverage; deposited at wFleaBase).
The main genes that were activated following the exposure of pear leaves to salt were predominately involved in basic biological processes, biosynthesis of secondary metabolites, signal transduction mechanisms, and other cellular components and molecular functions (based on their matches in the COG, GO and KEGG databases).
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based on their associate
based on their alignment
based on their equivalent
based on their competition
based on their matches
based on their ninja
based on their lack
based on their success
based on their experience
based on their year
based on their position
based on their history
based on their post-mortem
based on their gender
based on their judgement
based on their yield
based on their fitness
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