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We located the selected regions based on their locus ID and followed standard primer design guidelines, aiming for primers with a length of 22 25 bp, 40 60% GC content, melting temperature (Tm) = 55 62°C, the presence of a 3′ GC clamp, and without repeats, runs, or secondary structures such as hairpins, dimers, and cross-dimers.
For each demultiplexed sample, we computationally sorted reads based on their locus of origin and reaction type (E or G) by matching the start of read 1 to each of the 60 3' flank primers and the start of read 2 to each of the 60 5' flank primers (E reactions) or the L1-specific primer (G reactions) (Additional file 3: Figure S2).
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In addition, several genes were clearly adjacent to one another based on their loci.
Loci throughout the genome show different levels of neutral substitution between BY and RM due to ancestral recombination (see Text S1), so if cis-acting QTL are selectively neutral, we expect them to accumulate at a clock-like rate based on their locus-specific coalescence time.
Microsatellites can be assigned to estimated death rate classes based on their locus-specific probabilities for each class.
After eliminating the incomplete sequences manually, 159 sequences remained were finally identified as ZmPPs and renamed based on their loci on chromosomes.
In this study, we identified 136 WRKY proteins encoded by 119 WRKY genes from the maize B73 genome and named them from ZmWRKY1 to ZmWRKY119 based on their loci on corresponding chromosomes.
The software allows excluding and assigning putative fathers based on their multi locus genotypes.
Among the list of 376 genotypes obtained, the distinct genets were recognized based on their multi locus genotypes (MLGs) assessed with the 9 microsatellite markers following [ 58].
Individuals are then assigned probabilistically to one or more clusters based on their multi-locus genotype.
Captive individuals were assigned to island, species and, in many cases, population based on their multi-locus genotypes using two different approaches.
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CEO of Professional Science Editing for Scientists @ prosciediting.com