Sentence examples for based on their flux from inspiring English sources
Once an optimal flux distribution is computed via our MILP formulation, the localization of enzymes can be predicted based on their flux activity in various compartments.
BN analysis, in this study, was used to predict causal relationships among reactions and subsequently their metabolic modules based on their metabolic fluxes.
Using ratiometric flow cytometry to calculate the flux in each cell based on this ratio, we are able to not only quantify flux in individual cells but to sort cells based on their relative autophagic flux (Fig. 1B).
For instance, sorting for different levels of induced flux can be used to isolate cells that are either efficient inducers of flux in response to a given stimulus or poor inducers based on their levels of flux.
Based on their importance on the flux control and regulation of metabolic pathway, we built the first evidence-based database by systematic collecting RLEs in Human, Mouse, Rat, budding Yeast and E. coli[ 8].
This approach involves grouping functionally related reactions based on their genomic context and flux-converging pattern analyses.
Dialysis membranes are further classified based on their ultrafiltration coefficients into high-flux and low-flux membranes (i.e. for a given transmembrane pressure gradient, high-flux membranes have a higher filtration rate than do low-flux membranes).
We provide here a detailed protocol for our recently published method using mCherry-EGFP-LC3 to quantify autophagic flux by flow cytometry and to sort subpopulations of cells based on their relative levels of autophagic flux.
Another example is Flux Design, which selects reactions for up-regulation or deletion based on their correlation with the objective flux computed from EMs that contribute to the target product [ 15, 16].
We have therefore developed a method to measure autophagic flux by flow cytometry and have used it to successfully sort cells based on their relative levels of autophagic flux.
We detail here a protocol using tandem-tagged mCherry-EGFP-LC3 (C-G-LC3) to quantify autophagic flux in single cells by ratiometric flow cytometry and to isolate subpopulations of cells based on their relative levels of autophagic flux.
