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Molecules are separated based on their electrophoretic mobility due to the interactions with the silica surface.
Based on their electrophoretic mobility, these CWPs have been identified after sequencing the entire S. mutans genome [ 18].
Based on their electrophoretic mobility at low pH, α-/β-, γ- and ω-gliadins are distinguished [ 4].
LMW-GS can be separated into three groups, the B, C, and D subunits, based on their electrophoretic mobilities on an SDS-PAGE gel.
They assume unique positions along the channel's separatory axis based on their electrophoretic (μ EP) and dielectrophoretic (μ DEP) mobilities (Fig. 2).
The gliadins are generally monomeric proteins, divided into three groups, α/β-, γ- and ω-gliadins, based on their electrophoretic mobilities at low pH [ 2].
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In bread wheat, multiple protein alleles were discovered for each of the three Glu-3 loci based on their differences in electrophoretic mobility in SDS-PAGE [52], and these alleles differed in their effects on certain aspects of bread-making quality [53] [55].
LMW-GS can be classified according to different criteria, based on their primary structure, electrophoretic mobility in SDS-PAGE and N-terminal amino acid sequence.
Under certain conditions, membrane protein complexes can be separated using electrophoretic gels based on their intrinsic charge states alone, without prior dye treatment.
This step separates proteins based on their molecular weight using a vertical electrophoretic device with either Laemmli buffer [ 46] or Tris- Tricine buffer [ 47].
Through comparison of regions of interest containing mitochondrial events considered polarized or depolarized based on their red/green ratios, we observed a dependence of electrophoretic mobility on membrane potential, with higher membrane potential generally resulting in decreases in electrophoretic mobility, which is the opposite trend observed in previous bulk studies.
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