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The estimation of putative transcription start site of SlOFP20 is based on the tomato ESTs and cDNAs database (https://solgenomics.net/) as well as our RNA-seq data (SRA SRP090032; SRP090032).
Genetic linkage groups were compared to the physical maps based on the tomato genome version SL2.40 using MapChart 2.2 [ 44].
For qTy-p11, a total of 124 predicted genes were identified using Marker2sequence [ 35] based on the tomato genome sequence (Sol Genomics Network, SGN).
For all of them the expected 12 linkage groups were found with most markers in the order as expected based on the tomato sequence (results not shown).
The ACS3 peptide (KKNNLRL SFGKR MY) is based on the tomato ACS2 protein sequence and contains a single Met residue at the P+5 position (underlined); however, there is no recognized requirement for a hydrophobic residue at this position.
Due to lack of sufficient genome coverage (<1X) of the data (even over the euchromatin part of the genome estimated to be ~500 Mbp based on the tomato genome), the tobacco genome could only be partially assembled.
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Based on the current tomato genome sequence and annotations from related databases, including SGN and PlantTFDB, a total of fifty-nine tomato NF-Y genes were identified.
A cluster of disease resistance-like proteins is present near qTy-p11 (based on the cultivated tomato genome sequence).
SNPs were called using the Infinium chip cluster file based on the SolCAP tomato collection and a manual classification was implemented when the default clustering was not clearly defined.
Though our main purpose at this stage was to provide a bioinformatics environment for supporting genome wide investigations based on the reference tomato genome in physiological conditions, the platform architecture has been designed and is proposed as a model approach for similar efforts.
(Fun fact: if you plot a graph based on the Rotten Tomatoes score of each successive Paranormal Activity film, you will have drawn a steep hill).
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