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This assay, T-Plex real-time PCR, is based on the Tm discrimination of the amplified allele-specific amplicons in a single tube.
To investigate these questions, we designed a 3888-member combinatorial peptide library based on the TM domain of Neu (ErbB2) as a model RTK.
Annealing temperatures were chosen based on the Tm of the primers.
The annealing temperature was selected based on the TM values of the primers.
Heterozygotes could not be distinguished based on the Tm but only by the shape of the melt curve.
PCR conditions were the same as described above except that the annealing temperature was employed based on the Tm values of each primer.
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Zhang, Y. & Skolnick, J. TM-align: a protein structure alignment algorithm based on the TM-score.
Based on the tm-scores the expression values of genes of query samples are also classified typical or atypical for each of the reference tissues.
Some of the systems increased curation efficiency based on the time spent in TM-unassisted versus TM-assisted curation.
Formulations were first ranked based on the measured Tm.
Based on the single TM measurement, the whole TEG system has also been designed and tested on the bench test.
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