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'Fluorescence minus one (FMO)' controls based on the staining combination used for the experimental sample but in which one antibody was left out and replaced with its isotype control were also used to set sort gates correctly.
Briefly, an anti-VEGF-A goat polyclonal antibody (A-20; Santa Cruz) diluted at 1 : 50 was used as the primary antibody, and status of VEGF-A expression was classified based on the staining intensity and the percentage of positive-staining tumour cells as low expression or high expression (Tanaka et al, 2001).
Quadrants were generated based on the staining of isotype antibodies so that isotype control- stained cells were in the bottom part of the quadrant.
The immunostaining score (IS) was based on the staining intensity among these categories and on the percentage of stained cells.
The "percent reconstitution" shown in figures was based on the staining results of anti-CD45.2 and anti-CD45.1.
The optimum ratio was experimentally determined based on the staining signals (8 µM peptide in the Figure 6).
Similar(14)
Similar to the in vitro study, the cytoplasmic presence of most of NF-κB was observed in the untreated control (Fig. 4Bi), and nuclear translocation was induced by IL-1β based on the stain-filled nuclei (Fig. 4Bii).
The PicoGreen stain exclusion assay differentiates between membrane-enclosed and non-enclosed DNA based on the stain's inability to cross a lipid bilayer.
It remains controversial whether traditional endothelial-derived capillaries also service these tumor cells, with opinions based on the stain used, the observer, and the intratumoral location of the observation.
The stained sections were scored independently, based on the MET staining intensity, by one pathologist and two other researchers.
The results of β-catenin immunostaining were based on nuclear and cytoplasmic staining and those of CK7 and CK20 were based on the cytoplasmic staining of tumor cells.
More suggestions(17)
based on the infiltration
based on the patch
based on the jerk
based on the discoloration
based on the colouration
based on the reaction
based on the stainability
based on the colour
based on the spot
based on the tone
based on the color
based on the impregnation
based on the breed
based on the client
based on the randomization
based on the leader
based on the book
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