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inactive: physical activity < 30 min./day on 5 d./w, based on the SQUASH questionnaire, which was completed by 89 controls and 85 intervention participants.
For the diet and control groups, adherence was defined as <60 min/wk increase in moderate to vigorous activities (≥4 MET), based on the SQUASH questionnaire or, if missing, on the ActiGraph activity monitor data.
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Another method is based on the squashing of plant tissues on a nylon membrane [ 3] and subsequent elution of the little amount of DNA bound to the membrane for PCR amplification.
As early as 1500 bce the Maya had settled in villages and had developed an agriculture based on the cultivation of corn (maize), beans, and squash; by 600 ce cassava (sweet manioc) was also grown.
The Activity Questionnaire for Adolescents & Adults (AQUAA), is based on the Short Questionnaire to Assess Health-enhancing physical activity (SQUASH)[ 30].
This questionnaire contained items on smoking, BMI >24 kg/m and physical activity <150 minutes/week (2 questions based on the Short Questionnaire to Assess Health-enhancing Physical Activity, SQUASH) [ 15].
In summary, 154 component foods were direct matches based on the food names; 122 component foods were close but differed in either unspecified details (e.g., "summer squash" and "squash") or preparation (e.g., "raw onion" and "cooked onion"); 26 component foods were matched using the TDS mapping file (e.g., "lentils" and "white beans").
Recently, the nucleases Zucchini and Squash have been proposed as the 3' termini nuclease candidate based on the evidence that they are also located in germ plasm and have a similar mutation phenotype in a loss of transposon silencing [ 18].
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