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Firstly, a pilot experiment was performed based on the soybean reference genome sequences [ 16].
Additional SSR markers were developed based on the soybean genomic database Phytozome [ 20] and soybean SSR database BARCSOYSSR_1.0 [ 31].
The identification of repetitive elements was based on the soybean repetitive sequence database and annotation of soybean genome browser http://www.phytozome.net/cgi-bin/gbrowse/soybean/.
This interval was flanked by markers at positions 32,120,834 and 35,053,712 based on the soybean genome assembly version Glyma.Wm82.a1.
Based on the soybean AGL12-LIKE expression profile, it is tempting to speculate that their functions in floral regulation may have been lost.
Using this method, the authors [ 22] were able to determine median physical-to-genetic distance ratio in common bean to be ~120 Kb/cM (based on the soybean physical distance derived from the pseudochromosome assembly).
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Although some of the accessions in the USDA Soybean Germplasm Collection have been evaluated for their biotic and abiotic stress resistance, seed constituents and productivity during the past decades, knowledge of molecular variation, diversity, genetic architecture, and selection that may underlie the phenotypic variation in the soybean genome based on the entire soybean collection is unknown.
Based on the tetrad soybean genome structure, four copies of duplicated genes or four homeologous regions in soybean are theoretically expected.
The results are reported in real 2012 dollars, based on the average soybean price of $11.50/ bushel and seed cost of $52/ bag150,00000 seeds) in 2010 and 2011.
We constructed a TF database named SoybeanTFDB which is based on the identified soybean TF repertoire.
Each array consisted of unique probe sequences (50 60mers) based on the reference 'Williams 82' soybean sequence Glyma.Wm82.a1.v1.1 (www.phytozome.net) (Schmutz et al. 2010) and spaced at ∼0.5- to 1.1-kb intervals on average.
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