Scale-up fermentation was carried out in a 5-L bioreactor based on the shake-flask fermentation results, maximum CHA yield of 48.53 mg/L was obtained at an air flow rate of 2.0 ± 0.1 VVM and an agitation rate of 400 rpm.
Based on the shaking culture results, TB, a complex medium and MMM, a synthetic medium (Table 1) were used in mini-pilot scale fermentation.
Jaiswal and Wald (2010) developed a specific empirical model for estimation of earthquake fatalities based on the shaking intensity and the number of people exposed to earthquakes recorded between 1973 and 2007.
Based on the cultivations in shake flasks B. bassiana ATP-02 was cultivated in the TKI mineral medium with 5% sugar beet molasses.
Based on the cultivations in shake flasks B. bassiana ATP-02 was cultivated in the mineral TKI medium with 5% sugar beet molasses and were inoculated with 5×104 AC/mL.
Based on the results from the shake flask experiments, A. adeninivorans G1216/YIC104-thl-phaB and a modified yeast complex media were selected for the optimization of (R -3-HB pR -3-HBon.
Based on the pattern of shaking measured on distant seismographs, scientists at the National Earthquake Information Center believe that the earthquake occurred along a north-south fault, but they have not yet identified the fault.
The dynamic balancing is formulated as an optimisation problem such that while the shaking force balancing is accomplished through analytically obtained balancing constraints, an objective function based on the sensitivity analysis of shaking moment with respect to the position, velocity and acceleration of the links is used to minimise the shaking moment.
Based on the medium obtained from shake flask optimization experiments, a scale-up fermentation was conducted in a 5 L bench fermentor.
(The energy measure is based on both the shaking and its duration).
