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Free radical scavenging ability of the samples, based on the scavenging activity of 1,1-diphenyl,2-picrylhydrazyl (DPPH) free radical, was evaluated using the procedure described previously [ 29].
The trolox equivalent antioxidant capacity (TEAC) assay is based on the scavenging of 2,2'-azinobis- 3-ethylbenzothiazoline-6-sulfonic 2,2'-azinobis- 3-ethylbenzothiazoline-6-sulfonic 2,2'-azinobis- 3-ethylbenzothiazoline-6-sulfonic 2,2'-azinobis- 3-ethylbenzothiazoline-6-sulfonic 2,2'-azinobis- 3-ethylbenzothiazoline-6-sulfonic 2,2'-azinobis- 3-ethylbenzothiazoline-6-sulfonic
These results offer new insights into innovative strategies against H. pylori infection based on the scavenging of bacteria from the stomach using specific H. pylori chelating biomaterials.
The free radical scavenging activity of the extracts, based on the scavenging activity of the stable 1, 1-diphenyl-2 picrylhydrazyl (DPPH) free radical, were determined by the method described by Bracaet al., 2001 [18].
The free radical scavenging activity of the CM of B. subtilis IMV B-7023, based on the scavenging activity of the stable 2.2-diphenyl-1-picrylhydrazyl (DPPH·) free radical, was determined by the method described by Shimada et al. [14].
Antioxidant capacity was measured based on the scavenging of ABTS.+ radical cation by the sample in comparison to trolox standard [ 12].
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Based on the DPPH scavenging assay, EC50 values of the leaf extracts were 73 (P. triloba), 79 (C. latebrosa), 116 (G. truncata), and 144 μg DM/mL (D. curranii).
This assay is based on the measurement of the scavenging ability of antioxidants towards the stable radical DPPH relative to the DPPH scavenging ability of the water-soluble vitamin E analogue Trolox.
Antioxidant assay [ 27] is based on the measurements of the scavenging ability of compounds towards the stable 2,2-diphenyl-1-picrylhydrazyl radical (DPPH).
In conclusion, the wild-type C. glutamicum acquired oxidative stress-tolerance based on the increased ROS-scavenging activity by introducing the β-ketoadipate pathway gene clusters, which suggests that the intermediates of the β-ketoadipate pathway contributed to the acquired tolerance.
The scavenging activity was estimated based on the percentage of DPPH radicals scavenged by the following formula: % scavenging = [ ( A 0 - A s ) / A 0 ] × 100.
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