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These observations were confirmed in a second assay based on the quantitation of hydroxyproline in procollagen by reverse phase HPLC (Fig. 3k) and provided strong evidence that mTORC1 is a key signaling node involved in mediating the potent fibrogenic effects of TGF-β1.
CellTiter-Glo kit determines the number of viable cells based on the quantitation of adenosine triphosphate, which signals the presence of metabolically active cells.
On the other hand, this finding confirms the necessity of developing a comprehensive quality evaluation standard based on the quantitation of the active components and on novel analysis technology to ensure the quality of DB medicine.
It is based on the quantitation of cellular ATP using the luciferin-luciferase reaction to produce bioluminescence [23].
Peptides were ranked based on the quantitation of tumour, kidney uptake and retention in the tumour during 4 h.
The CellTiter-Glo luminescent cell viability assay (Promega, Mannheim, Germany) is based on the quantitation of ATP, reflecting the presence of metabolically active or viable cells.
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The calculations were performed by the AlleleSEQR Quantitation Software based on the relative quantitation method (2-ΔΔCT).
Relative cell viability was evaluated by CellTiter-Glo™ Luminescent Cell Viability Assay (Promega Corporation, Madison, WI) based on the ATP quantitation representative of metabolically active cells.
In conclusion, the comprehensive analysis of blood serum metabolites by NMR using two different sample preparation methods was quantitatively evaluated based on the identification and quantitation of aqueous metabolites.
We additionally used a translation efficiency metric based on this quantitation to analyse potential associations with virus replication rate (Table 4) along with other variables (dinucleotide composition, CB bias, CAI, ENc and G + C content).
Since the developed assay is based on the detection and quantitation of rapidly replicating phage DNA, we first studied some parameters of lysis and propagation rates of the three phages including mean diameters of plaques, dynamics of bacterial lysis, burst size and duration of lytic cycle.
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