Sentence examples for based on the primers from inspiring English sources

No H2A.Z.1 equivalent variant was identified, but the apparent absence of H2A.Z.1 in C. carpio is likely to be the result of the methodology used by Simonet et al. to identify H2A.Z, which was based on the primers generated from only one of the zebrafish H2A.Z counterparts.

The 16S rRNA obtained based on the primers 5′-GCGGTTGTTACAGTCAGATG-3′ and 5′-GCCTCAGCGTCAGTATCG-3′ was used as an internal standard.

In order to develop rapid and sensitive methods for CPIV3 detection in infected goats, a TaqMan RT-qPCR was established in this study based on the primers and probe designed to amplify a 150 nucleotide-long region located within the M gene of the virus.

The degenerate primers used in this study were previously reported by Abraham et al. 2003 [7] based on the primers initially reported by Welschof et al. 1995 [26].

The UP (universal primer) primer was designed based on the primers of suppression PCR [ 25].

We used Bioperl to generate virtual PCR fragments for each SNP allele based on the primers designed, and to find restriction endonucleases that would differentially cut the fragments, thus creating molecular markers.

A 626 bp band corresponding to a vitamin C transporter (SVCT2) based on the primer design was detected by RT-PCR analysis in all breast cancer cell lines.

Taken together, when we introduce the contributions of Dr. Sanger in DNA sequencing, we really have to add the information that based on the primer-extension approach developed by Dr. Wu, and Dr. Sanger further refined the methodology and played an important role in DNA sequencing.

The specificity is mostly based on the primer mismatch at the 3′ end to fungal and plant sequences (Table  2).

PSY1 expression was evaluated based on the primer pair PSY1_F1R1 (amplification product of 218 bp) designed on the fourth exon.

However, the success of a PCR or qPCR assays is based on the primer pair design and its efficient evaluation.