Sentence examples for based on the primer from inspiring English sources

A 626 bp band corresponding to a vitamin C transporter (SVCT2) based on the primer design was detected by RT-PCR analysis in all breast cancer cell lines.

However, the success of a PCR or qPCR assays is based on the primer pair design and its efficient evaluation.

The specificity is mostly based on the primer mismatch at the 3′ end to fungal and plant sequences (Table  2).

PSY1 expression was evaluated based on the primer pair PSY1_F1R1 (amplification product of 218 bp) designed on the fourth exon.

Each subsequent 20 μL of PCR contained 50−100 ng template and was based on the primer pairs listed in Table S1 and standard PCR conditions.

Based on the primer design, the expected amplification sizes were 756 bp (AcCRa), 832 bp (AcCRb), 512 bp (AcCRc) and 224 bp (actin).

Taken together, when we introduce the contributions of Dr. Sanger in DNA sequencing, we really have to add the information that based on the primer-extension approach developed by Dr. Wu, and Dr. Sanger further refined the methodology and played an important role in DNA sequencing.

The 16S rRNA obtained based on the primers 5′-GCGGTTGTTACAGTCAGATG-3′ and 5′-GCCTCAGCGTCAGTATCG-3′ was used as an internal standard.

The degenerate primers used in this study were previously reported by Abraham et al. 2003 [7] based on the primers initially reported by Welschof et al. 1995 [26].

Primers and Taqman® probes for the selected set of genes were chosen using Primer Express software (Applied Biosystems, Foster City, CA, USA) based on the primer-probe combinations prioritizing the 5' end of the transcript and in which the sense and anti-sense primers corresponded to 2 different exons separated by an intron of at least 500 bases.

The UP (universal primer) primer was designed based on the primers of suppression PCR [ 25].