Sentence examples for based on the polymerase from inspiring English sources
The method is based on the polymerase chain reaction (PCR) used in conjunction with restriction fragment length polymorphism (RFLP) analysis and comprises three parts.
The assay was based on the polymerase chain reaction (PCR) with real-time fluorescence resonance energy transfer (FRET) measurements to amplify cDNA products reverse transcribed from mRNA.
A method based on the polymerase chain reaction has been developed for differentiating between genotypically and phenotypically distinct strains of Giardia duodenalis and quantifying the amount of initial template of the different genotypes in mixed populations.
This scheme is based on the polymerase chain reaction and utilizes a degenerate stem-loop annealing primer (dSLAP), consisting of a stem-loop structure followed by 12 random nucleotides, and is called the C-ORF (complete open reading frame) technique.
Objectives: The aims of the study were to determine whether a Gram stain of cervical mucus can accurately rule out infection with Neisseria gonorrhoeae or Chlamydia trachomatis and to compare a diagnostic test that is based on the polymerase chain reaction with a deoxyribonucleic acid probe in the detection of these organisms.
In addition to NGS, today's medical experts also use assays based on the polymerase chain reaction (PCR).
In the case of influenza virus, extrapolation of the template from the observed duplex region, based on the poliovirus polymerase elongation complex model (Gong and Peersen, 2010), would result in the 3′ end overshooting the polymerase active site by three nucleotides as previously discussed (Reich et al., 2014).
A high-resolution method for genotyping of human leukocyte antigen-B gene (HLA-B) based on the optimized polymerase chain reaction (PCR) cloning and sequencing procedure was established.
Primers and probes for the NLV Basic Kit assay were based on the RNA polymerase region of the prototype NLV, Norwalk virus (NV) genome and could consistently detect 104 RT-PCR detectable units of NV RNA in a stool filtrate.
The PCR master mix was based on the AmpliTaq Gold DNA polymerase (Applied Biosystems).
Other efforts to develop rapid tests for recreational water quality are based on the quantitative polymerase chain reaction (for example, see EHP 114:24–28 [2006]).
