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A set of 11 PCR primer pairs (Table S2) was based on the pol and nap mitotype sequences, of which eight (P3-9, P11) are pol-specific.
In particular, we performed a comprehensive phylogenetic analysis of all families based on the pol polyprotein (see methods).
We found strong evidence that γ1 ERVs from Eurasian and African species based on the pol and env phylogenies belong to two different lineages.
These vectors differ based on the Pol III promoters used to drive the gRNA: AtU6-26p in pBUN401-ZT1, OsU3p in pBUN411-ZT1 and TaU3p in pBUN421-ZT1.
The HIV-1 subtype was determined using the same env fragment, and this confirmed the original subtype classification based on the pol gene.
This number of novel non-B/B recombinants is almost certainly an underestimation as the initial selection of non-B infections was based on the pol subtype and excluded infections classified as subtype B in pol.
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This study used a survey based on the one developed by Poling and Evans (2004).
In this paper we describe a robots design course based on the didactic technique POL (Project Oriented Learning) where the students identify an opportunity area for a robot project application, propose a design, and build a working prototype.
This latter concept is based on the fact that Pol III can catalyze transcription reactions in a simplified in vitro transcription system in the absence of TFIIIB and TFIIIC that facilitate reinitation of transcription [17].
An alternative model is based on the hypothesis that Pol ε has three distinct roles in replication.
The three phylogenetic trees, based on the well conserved Pol domains or on more divergent Gag and Env domains, show the same topology, supported by high bootstrap values.
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CEO of Professional Science Editing for Scientists @ prosciediting.com