Sentence examples for based on the pcr from inspiring English sources

This typing method is a modified mixed-linker PCR, a rapid approach based on the PCR amplification of HhaI restriction fragments of genomic DNA containing the 3′ end of IS 6110 and resolving the amplicons by polyacrylamide gel electrophoresis.

FLiP is a modified mixed-linker PCR, a rapid typing method based on the PCR amplification of RFLP fragments containing the 3′ end of IS 6110 and resolving the amplicons by polyacrylamide gel electrophoresis [ 17].

Based on the PCR amplification patterns and DNA sequences of the cloned fragments, it was noted that the protease gene profiles of the three distinct strains of B. thuringiensis subsp. kurstaki HD73, tenebrionis and israelensis T14001 are varied.

The method is based on the PCR amplification of species-specific DNA fragments using fluorescent oligonucleotide primers, which were designed based on sequence divergence within the internal transcribed spacer region of nuclear ribosomal DNA.

The assay was based on the PCR amplification of 18SrDNA gene fragments from metagenomic DNA preparations, and it resulted in the amplification of detectable products for all sampled sites.

In this work we describe rapid, sensitive and robust assays based on the PCR technique to discriminate the main species included in section Nigri: A. japonicus, A. heteromorphus, A. ellipticus and the two morphologically indistinguishable species of the A. niger aggregate: A. niger and A. tubingensis.

This study developed a simple method to obtain numerous 5′-UTR variants and analyze their translation efficiency based on the PCR-based site-directed mutagenesis and the expressional PCR using coupled in vitro transcription/translation system derived from Escherichia coli and eGFP gene as a template.

A band of approximately 3.4 kb was detected, which is close to the size of the predicted full-length SSIV transcript based on the PCR-amplified fragments.

Shigella spp. was isolated from a feces sample obtained from the patient on illness day 8, and an STEC signal (based on the PCR-positive stx 2 gene) was detected from the same sample.

In this context, the present work represents an effort to identify BPV types employing an alternative screening method based on the PCR-RFLP technique and correlating the histological data of the analyzed lesions with the diagnosed viral type.

Sequence analysis based on the PCR-amplified products (primer sequences were removed) indicated that among the 47 HEV strains, 31 belonged to genotype 4, 13 belonged to genotype 1, and 3 belonged to genotype 3, which indicated that genotype 4 is the main type prevalent in humans in eastern China.