Based on the nucleotide sequence of a SCAR fragment of CPA-2, one primer set and TaqMan MGB probe were designed.
Based on the nucleotide polymorphisms observed between each Hd6 region, we examined the phylogenetic relationships among the varieties and species.
Specific forward primers for other anisakid species were also designed based on the nucleotide sequences of the ITS region.
Two sets of primer probe pairs were designed based on the nucleotide (nt) sequence of the nucleocapsid (N) gene.
We have developed a system to classify them based on the nucleotide differences in SC genes (coa).
Based on the nucleotide sequence of dal, the primers dal-F/dal-R were designed to amplify the fragment dal using the B. subtilis 168 as the template.
Based on the nucleotide variations, a total of 17 single nucleotide polymorphisms (SNPs) and five insertion/deletions (indels) of Gn1a were detected in O. sativa.
Alleles are defined based on the nucleotide sequence encoding the ERF and not flanking or intervening sequences, which have additional polymorphisms (S. Heuer, personal communication).
Phylogenetic analysis based on the nucleotide sequences showed different grouping of the HKT family genes as compared to the prevailing protein sequence based classification.
Based on the nucleotide sequence of 16S rRNA genes, the toluene-oxidizing bacteria in microcosms were identified, i.e. Ralstonia sp. P-10 and Pseudomonas putida.
A resulting dendrogram was constructed, which was based on the nucleotide sequence data of 12 UPTC isolates and demonstrated that the UPTC were genetically variable.
