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Based on the nuclei position in confocal analysis, the DEL cells were depleted from the leading edge region at 8 hpf (Figure 3B) relative to YSL and EVL nuclei.
The somatic ploidy level was determined using DNA flow cytometry (Epics Altra, Beckman) based on the nuclei extraction method of Galbraith et al. (1983) [ 67].
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For each gene and each method, the estimates for ν are based on the per-nucleus or per-nucleus-pair estimates pooled across all embryos involving that gene.
Next, the corresponding 359 or dodeca FISH image was masked based on the identified nuclei, and FISH signal intensity was enhanced to increase the contrast between staining and background.
A stochastic model developed based on the Single Nucleus Mechanism and a deterministic population balance were used to theoretically study the effect of volume on MSZW.
The study's results indicate steroids, based on the estratriene nucleus, can modulate lipid packing as evidenced by (1) decreased membrane fusion events and (2) decreased membrane fluidity.
The dependency on volume of the MSZW cannot be explained by the deterministic population balance model but can be explained by the stochastic model based on the single nucleus mechanism.
A new class of chiral ligands, e.g., -9, based on the benzoxazine nucleus, has been designed and synthesized in three steps from the commercially available starting materials salicylamide and -menthone.
The structures of porphyrins, chlorines, and phthalocyanine are based on the tetrapyrrole nucleus, whereas the others have different molecular frameworks [ 21].
Consequently, we subsequently quantified maternally derived myofiber nuclei based on the morphology of the nucleus and its location within the fiber (Fig. 2, bottom).
We experimented with various algorithms based on the distance between nuclei, as well as the intensity of the image between nuclei.
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