The resultant contigs were joined into scaffolds based on the mate pairs information and the scaffolds were clustered using TGI Clustering tools [ 47].
Every evening, the identity of the males to observe the next day was determined based on the mate-guarding activity of each male and on whether they were followed or not that day.
The objectives of this study are the evaluation of the matching approach that was previously adapted to include (a) using the MATE as the standardized intake assessment, (b) a treatment recommendation for each patient based on the MATE results, and (c) a feedback session with the patient.
For the patients in the IG, this includes (a) the LOC that is recommended based on the MATE, (b) therapists' dissenting recommendations, if they occur, and reasons for the disagreement, (c) results from the feedback session, and (d) the final referral decision that was made at discharge.
The data gathered then allowed us to modify broodstock and juvenile production protocols based on the species' mating system.
Parental allocation was performed using PAPA software [ 15] based on the known mating data to increase the accuracy of parental assignments [ 8].
In the second (GSD + MA), from the 6250 (25 × 250) possible matings, we chose the best 250 based on the prediction of the mating (Gij), and we generated four new individuals for each mating mate.
As expected, the transcripts produced by GAL1pro:: a1 were translated based on the a/α diploid mating phenotype of MAT α, HMR GAL1pro:: a1, sir4 ∆ strains when grown on galactose-containing medium.
Overall, then, while we currently cannot form a causal link between competition and conflict, the existence of strong male-male and male female interactions matches our expectations of how these phenomena should emerge based on the structure of the mating system within this species.
Third, medaka shapes mating preferences based on the color of tank mates which they grew up with (see Methods).
