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This proposed safe level is based on the LOQ for the assays developed by US FSIS.
Our comparison is based on the LOQ of the assay rather than on the lower limit of detection [ 7, 28].
The concentration ranges of the five-point calibration curves were 20 1200 μg/kg for DON and between 10 and 250 μg/kg for 3Ac-DON, DON3Glc, HT2, NIV, NIV3Glc, T2 and ZEN, with the lowest calibration point based on the LOQ values of each analyte.
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The major criterion to determine the LOQ is based on the precision.
For compounds not detected in the blanks, the LOQ was calculated based on the signal-to-noise ratio 10/1.
ICH guidelines were used for calculation of LOD and LOQ [11], based on the intercept standard deviation and slope of the calibration plot through the following equation.
The LOD and the LOQ were statistically determined based on the t 99 S LLMV method where t99 n-1) = 3 (Corley 2003).
The LOD and LOQ were calculated based on the chromatographic responses of the analytes as the average plus three and six times the standard deviation, respectively.
LOD and LOQ were calculated based on the standard deviation of the responses of the lowest concentration in calibration curve of six runs (n =5) and the slope of the calibration curves of external standards, respectively.
LOD and LOQ of the assay were determined based on the signal/noise (S/N) criteria and were found to be 0.20 μg mL−1 (S/N = 3) and 0.65 μg mL−1 (S/N = 10), respectively.
Exposure levels reported in Table 2 are based on the third analysis excluding samples with values below the LOD or below the LOQ.
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