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The last four columns show the function of the most similar protein as identified by blastp (based on the locus' protein sequence) in the Uniprot database, followed by the accession number of the hit, the identity on protein level and the taxonomy of this entry.
H. pylori has been assigned many alleles of Type I specificity subunits, from hsdS1 to hsdS6, based on the locus and sequence similarity of the entire ORF (open reading frame) [33].
In this study, the interpretations of the potential outliers are based on the locus effect (αi).
Primers were designed based on the locus sequences of A. nidulans FGSC A4 obtained from The Broad Institute's homepage (www.broadinstitute.org).org
The Gene Ontology annotations were assigned for each unigene based on the locus identifiers using GO annotation and categorization tool at TAIR.
We found that the requirement for a minimal 75% overlap between coding regions yielded conservative values closest to those obtained from the classification based on the locus ID annotation from the RefSeq database (supplementary table S1, Supplementary Material online).
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The method employs a three-axis CNC milling machine equipped with suitable ball-end cutter and is based on the locus-tracing concept.
This mode is designed for test crosses and creates a catalog of possible loci based on the loci present in the parents.
The GTR + G + I nucleotide substitution model was used to infer the species tree based on the loci listed above and ToxB tree based on ToxB homologs.
Thus, homoeologous loss does not appear to occur instantaneously upon hybridization or polyploidization in Tragopogon, at least based on the loci examined thus far.
A VNTR-typing scheme based on the loci established on M. bovis isolates from animals (8 ) would detect M. bovis clusters derived from foodborne outbreaks or horizontal transmission of disease between humans in Ireland.
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