Sentence examples for based on the ligation from inspiring English sources
This technique is based on the ligation followed by PCR amplification of two adjacently hybridising oligonucleotides.
In this expanded method denoted as ATLAS (A-dvanced T-elomere L-ength A-nalysis in S. cerevisiae), we have introduced: 1) set of new primers annealing with high specificity to telomeric regions on five different chromosomes; 2) new approach for designing reverse telomere primers that is based on the ligation of an adaptor of a fixed size to telomeric ends.
Briefly, the method is based on the ligation of oligonucleotide adaptors.
Recent modular procedures based on the ligation of segments provide the best overall yields.[ 5a, b] However, the modular character introduces extensive purification procudures, thus making the procedures unsuitable for the automated parallel generation of reagents.
In this study, a label-free fluorescence strategy for sensitive detection of ATP based on the ligation-triggered super-sandwich is reported.
The analysis of bleeding events after esophageal ligation was based on the location of bleeding, the indication for EBL (Table 3).
Based on the post ligation yield of PTEN protein it is estimated that there is a maximum PTEN-thioester concentration of ∼80 μM in the ligation reaction.
In 2007, Applied Biosystems (ABI) introduced the SOLiD second-generation sequencing system based on the oligonucleotide ligation technique and two-base encoding system, whereas Illumina released Solexa sequencing technology.
Using Hi-C analysis (a biochemical approach based on the proximity ligation procedure), Dekker and collaborators also came to the conclusion that both open (active) and closed (repressed) chromatin domains were composed of a single type of fiber.
A further development of the RespiFinder-19 assay is the RespiFinder-SMART-22 assay, which is also based on the multiplex ligation-dependent probe amplification.
Moreover, using the BeeDoctor diagnostic tool [ 17] which is based on the multiplex ligation-dependent probe amplification technology, we were also able to screen in parallel for the negative-strand intermediate.
