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To refine our IS annotations (i.e., to identify fragments and highly divergent copies that may have been missed before), we generated a library of Wolbachia IS sequences based on the IS elements detected as described above.
Based on the IS analysis, the hydrogen diffusion coefficients of those three components were successfully calculated and compared with the diffusion coefficient in other systems.
In addition, although our model was based on the IS success model (a causal model asserted by DeLone and McLean), one must be careful when making such a causal declaration [ 70].
For all culture-positive cases, we used the standardized methodology to genotype the corresponding M. tuberculosis isolates by restriction fragment length polymorphism, based on the IS 6110 insertion sequence (IS 6110-RFLP)[ 14].
In this study a transposon library of the completely sequenced type strain ATCC 13032 with a statistically representative size was constructed using an transposon vector based on the IS 6100 element.
Based on the IS 1245 or IS 1311-RFLP patterns, various clades of M. avium and its subspecies could be distinguished, related to infections in birds, pigs, and humans [ 79– 81].
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