Second, the porcine miRNAome along the entire intestine (duodenum, proximal and distal jejunum, ileum, ascending and transverse colon) was unraveled using customized miRNA microarrays based on the identified sequences as well as known porcine and human ones.
Selection of representative genes depended on success of the primer design based on the identified sequences.
Based on the identified sequences we constructed for each of the groups a TAR cloning vector.
Functional domains are easily extracted based on the identified sequences using a randomly primed prey library as a non-biased-representation [19], [26].
Based on the identified unique sequences and the subcellular localization of relevant proteins, a C4-like photosynthetic pathway was found to exist in M. incisa that enables this microalga to survive under low ambient CO2[ 38, 68].
At first, we determined the protein length based on the identified amino acid sequence (Additional files 5, 6, 7, 8 and 9), because the Tomato Genome Consortium (2012) reported a similar amino acid sequence length for ~43% of tomato proteins with their respective Arabidopsis orthologues.
Based on the previously identified sequence information, different sizes of BjMTP1 promoter regions were PCR amplified using high fidelity platinum Taq DNA polymerase with 5' PstI- 3' BamHI promoter specific primers.
The isotype of each sequence is determined based on the identified constant region primer and then 100,000 sequences is randomly selected to be aligned to VH germline genes using IMGT/HighV-Quest.
Based on the identified segment pairs on the coding sequence level, we focused on a subset of transcript sequences of equal size in both libraries to uncover temperature-related patterns in the codon usage.
Based on the identified structural templates and the corresponding sequences, several 3D models were constructed using Modeler module in Discovery Studio 2.0 (Accelrys Software Inc., USA).
