Sentence examples for based on the hplc from inspiring English sources
A standard curve was then created to calculate the CHIs of all iodo-PARPi based on the HPLC retention time.
Third, based on the HPLC reference method, a NIR model able to quantify the API in the drug reservoir was developed and thoroughly validated.
Based on the HPLC chromatogram, the linker dimer 3 accounts for only 20%, and there is negligible formation of protein-linker dimer from linker dimer 3 and cys-anxA5, 5 according to SDS gel electrophoresis (Figure 5).
The ratio of 3-I to 3-II is about 7 1 based on the HPLC analysis.
As we reported earlier [ 9, 23], based on the HPLC chromatogram data, two peaks were represented as pure inositol hexaphosphate.
The presence of flavonoids in MEMC was also expected based on the HPLC analysis wherein some of the peaks detected in the UV spectra represent flavonoid-based compounds.
Moreover, the visible light photoelectrocatalyic degradation pathways of PEN G were proposed based on the HPLC-MS/MS measurements, which included decarboxylation and oxidation of the target organic, and finally mineralization to H2O, CO2, NH4+ and NO3−.
The least polar NCC (42.8 mg, 21.3 %) was identified as re-isolated starting material (4 epi ), based on the HPLC-retention time, its UV spectrum and a pseudo molecular ion at m/ z 645.2 [ M+H]+ indicating the molecular formula to be C35H40N4O8.
The crude product was lyophilized, redissolved in 50.0% aqueous acetonitrile containing 0.1% trifluoroacetic acid and purified by semi-preparative reverse phase HPLC using a C18 Gemini (Phenomenex, Torrance, CA, USA) column (10 × 250 mm) at a flow rate of 5 mL·min−1 and eluted using an appropriate gradient based on the analytical HPLC profile.
Radiochemical yields were calculated based on the Radio-HPLC collections.
Considering the methodological limitations of the quantification of a microdose amount of F-florbetaben (i.e. estimation based on the radio-HPLC and the total F radioactivity in plasma), the estimated concentrations and derived pharmacokinetic parameters provide only explorative and orienting information.
