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However, based on the GUS activity, the difference was observed to be ~110 fold.
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The transformation frequency can reach 92% based on the detection of GUS activity.
Thus we define our Phase- Coupled Meta-analysis (PCM) score for a gene g as the magnitude of a sum of L g (≥ 5) vectors, PCM g) =, where the jth vector ⟨ M gi, θ gi ⟩ of g has direction θ gj given by the phase angle of g in experiment j and magnitude M gj defined as M gi = exp (qP gi ) × exp qE gi ) based on the percentile scores described above.
Based on the observation that BAS1-GUS expression is present in the shoot apex and that the early-flowering phenotype of bas1-2 sob7-1 is dependent on the presence of functional phyB, we hypothesized that phyB signaling is regulating BAS1 expression in the shoot apex which in turn affects flowering time.
For pesticides the leaching potential is determined by calculating the Groundwater Ubiquity Score (GUS), based on the sorption coefficient and soil half-life for each pesticide compound.
In this report we describe two dual reporter systems that between them meet the above criteria: the first is based on the reporter genes NAN and GUS [35], [36], for both of which fluorogenic and histochemical substrates are available; the second on the genes coding for GST and GFP [37], [38].
This finding is based on the experiments comparing the expression of gus gene, encoding the synJ as 5′UTR under the control of 35S promoter with expression cassettes based on vectors like pBI121 or pRT100.
Isogenic prfA* mutants constructed via allelic exchange were isolated based on the PrfA*-dependent increase in actA-gus-neo expression that enabled selection for prfA* colonies on selective media containing neomycin and 5-bromo-4-chloro-3-indolyl-β-D-glucuronic acid (x-gluc), a substrate for GUS activity.
Metal concentrations were chosen based on the concentrations that produced the maximal increase in GUS activity in transgenic B. juncea containing a BjMTP1 promoter::GUS construct (see below).
Based on the homology to Drosophila and other organisms, we identified the GUS-related genes in the cDNA library constructed with the previtellogenesis ovary tissues.
Three shRNAs were chosen based on the sequence of the mouse RANK gene (GenBank gi: NM_ 009399).
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