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Mendelian error detection, gender estimation and population-based checks, based on the genotyping data, can identify sample mix-ups, pedigree misspecifications and sample contamination.
A core set of 6000 SNPs (BARCBean6K_3 BeadChip) with high genotyping quality and polymorphism was selected based on the genotyping of 365 dry bean and 134 snap bean accessions with the BARCBean6K_1 and BARCBean6K_2 BeadChips.
Based on the genotyping data from 119 SSR markers that were evenly distributed across all 12 chromosomes, population structure was estimated with the model-based (Bayesian) cluster software STRUCTURE 2.2 (Pritchard et al. 2000).
These two genotypes are also believed to represent different genetic backgrounds based on the genotyping results.
Out of these 9727 calls, 6886 are homozygous based on the genotyping chip data.
The remaining 73 (4%) strains could not be classified based on the genotyping techniques used (Additional file 2: Figure S1).
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Then, we used MAS to select 23 BC1F1 plants carrying one or two target chromosome segments, based on the genotypes of 86 DNA markers distributed across the genome.
A scoring system was developed to determine the minor contributors in DNA mixtures based on the genotypes identified using MPS.
Using hybriddetective we simulated multigenerational (viz. pure wild, pure farm, F1, F2, and backcrosses to wild and farm) hybrid datasets based on the genotypes of our wild and farmed baselines at the 95 SNPs in our panel.
A clear separation based on the genotypes site of origin among the genotypes for drought tolerance could not be established when analyzed using principal component analysis.
The genotypes of the hybrids were deduced based on the genotypes of the parental lines.
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