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A real-world example motivates the case study, based on the downstream oil supply chain of mainland Portugal, which is used to validate the applicability of the stochastic model.
Operational constraints include maximum non-damaging flows and reservoir release, rate-of-change (based on the downstream channel's ability to accept decreasing amounts of water without bank sloughing), reservoir Guide curves for reservoir operation.
MLCK promoter activity was determined by EMSA, using a duplex oligonucleotide probe based on the downstream NFκB-binding element in the MLCK promoter region (Ye et al, 2006).
To be complete and effective, following the first step of "genetic personalization," individuals in each subgroup must be characterized based on the downstream response of that genotype.
Two ~100 bp spaced gene specific reverse primers (GSP1 and GSP2) for each target, designed based on the downstream sequence of the miRNA:target binding site at the target gene sequence.
Therefore, the age estimated based on the downstream SNPs seems to reflect the real age of ADH1B*47His allele, which is also consistent with the ages estimated from the STR variations.
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Based on the apparent downstream and upstream convergence of these dated terraces, we identify a doming structure or anticline within the basin, which results in a tilt rate of 10−6 to 10−7 per year in the lower Erhjen River valley.
(A ) Relative occupancies based on the codon downstream of the inferred A site, compared to the A-site occupancies as in Figure 5. Similarly, small footprint abundance (B ) and large footprint abundance (C ) for the inferred A site and downstream codon.
PCR primers were based on the upstream and downstream sequence of the coding region of the Pm3b allele.
For each genomic locus, we designed primers based on the upstream and downstream sequences surrounding the predicted integration sites.
Differentially expressed transcripts were validated based on the protocol for downstream analyses of de novo assemble using Trinity (see section Methods).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com