Sentence examples for based on the confluence from inspiring English sources
Cell confluence was evaluated by IncuCyte ZOOM™ 2013A software (Essen BioScience, UK) based on the confluence masks as recommended by manufacturer.
Transduction with both types of vector was about equivalent both in proliferating (Figure 1, No NGF) and differentiated (Figure 1, Plus NGF) cells and, based on the calculated MOI and percent confluence, about 50% as efficient for both types of vector particles as on MDTF cells.
For all simulations, the confluence percentage was set to 77%, based on the average empirically-measured value in mesendoderm explant images [ 4].
Cells were inoculated the day before transfection, which was performed at ~60% cell confluence using Lipofectamine 2000 (Invitrogen) based on the manufacturer's instructions.
The study showed that the confluence of river Niger and river Benue can be developed based on the methods outlined above and that the project can be a great benefit to tourists, investors and the government.
The Bismuth classification is based on the localisation of biliary strictures according to the distance from the biliary confluence but does not encompass the entire spectrum of bile duct injuries [ 31].
The subsequent maps based on the knowledge he provided fail to indicate that he had reached a large island at the confluence of the Ottawa and St. Lawrence rivers.
When culture plates achieved ~90% confluence, RNA was extracted and purified following TIGR standard operating procedures based on the Trizol method (Invitrogen) [ 30].
Confluence was estimated by phase microscopy (Olympus CK-40; Olympus Cor., Tokyo, Japan) based on the occupied surface of the tissue culture flask, and MSC density was determined by microscopic enumeration with a hematocytometer (Marienfeld, Germany).
At 75% confluence, cells were harvested by using 0.25% trypsin and were sub-cultured into 75-cm2 flasks, 6-well plates, and 96-well plates based on the type of experiment to be conducted.
