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As a control, we also analyzed the occupancy based on the codon one position 3′ of the A site, which has not yet entered the decoding site.
In B. subtilis the proportion of the tRNAs pairing to more than one codon is calculated based on the codon usage ratio.
The optimization was carried out based on the codon usage frequency of the closely related strain Aspergillus niger (Abarca et al. 2004).
The gene encoding the d-hydantoinase from A. crystallopoietes DSM 20117 was optimized based on the codon bias of E. coli for improved soluble expression using the GeneArt™ software.
These sites were used to clone in-frame all Plasmodium synthetic cDNAs designed based on the codon bias of C. reinhardtii.
Ks was estimated based on the codon alignments.
Similar(24)
Therefore, we sought an alternative way to compare zinc finger sequence similarity and thus DNA binding specificity: We computed ZNF motif matrices for each 8q24.3 ZNF gene based on the codon-aligned individual zinc finger sequences (see Methods).
This was attributed to the fact that S is based on the codons for only four amino acids, which did not include codons that were enriched in the highly expressed genes of P. aeruginosa [ 8].
A similar analysis of asymmetric methylation, based on the codons CHG and CHH (H = A, C or T) was carried out by assessing the frequencies of N| NN and NNN, and a more obvious alternation in C| NN and NNG frequencies than in others was found based on both intron number and exon position (data not shown).
We decided to optimize the TcTrx DNA sequenced based on the yeast codon usage table (The codons were optimized by using the Codon Optimization Tool provided by the Integrated DNA Technologies (http://sg.idtdna.com/CodonOpt) with codon usage table of Saccharomyces cerevisiae.
Figure 1 Nucleotide sequences of TcTrx cDNA and its codon usage optimization based on the yeast codon usage table.
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based on the high-resolution
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