Sentence examples for based on the assembled from inspiring English sources
Novel internal exons were defined based on the assembled contig clusters sharing at least one exon of annotated transcripts, and were further required to fully reside within the intron region of this annotated transcript.
Novel transcribed elements of annotated genes, including novel internal exons, novel splicing donor & acceptor splicing sites and novel 5′UTR & 3′UTR extensions, were defined based on the assembled contig clusters overlapping with at least one transcript, annotated by the Ensembl database (version 64).
The sequence of the virus which showed 83% identity with DsMV infecting Zantedeschia aethiopica (China) was confirmed through amplification and sequencing with primers designed based on the assembled sequence.
The primers that were designed with Primer software (version 5.0) based on the assembled transcriptome were used for amplification, and the annotations of the products are listed in Table 1.
Finally, Vector NTI v9.0 was used to generate maps of the mt genomes based on the assembled sequence data.
Primers (Additional file 12) complementary to the genes were designed based on the assembled P. cactorum unigene sequences.
APHENA was based on previously assembled databases in the first-stage analysis.
The assembly of genomic sequences from C. ulcerans BR-AD22 led to 25 large and 3 small contigs, based on 178,615 assembled reads representing the total number of 59,757,327 sequenced bases.
For comparison, QDSSCs based on the randomly assembled nanosheets-based microspheres (RATSs) and commercial P25 nanoparticles were also assembled.
Separate Velvet assemblies were also run with a fixed low and high word length, and the best assembly was chosen from the three trials based on the total assembled length of contigs.
The annotations made from each sequencing data were assembled by Cuffmerge, and fragments per kilobase of transcript per million mapped reads (FPKM) was measured using Cuffnorm algorithm based on the newly assembled annotation55.
