Sentence examples for based on the adaptor from inspiring English sources

were based on the adaptor sequences.

To generate 3' cDNA templates for 454 sequencing, the 3' cDNA was amplified by PCR using the sense primer based on the 5' adaptor sequences and the antisense primer based on the 3' end university adaptor seqeunces.

To account for adaptor trimming and sequence quality in this assembly, raw sequence files (SFF format) were modified based on the adaptor trimming information described above, and these modified SFF files processed with Newbler.

The enriched transcripts were converted into cDNA by using the biotinylated primer based on the universal adaptor sequence at the 3' ends of transcripts.

The enriched RNA was converted into double-strand cDNA by using a cDNA synthesis kit (Invitrogen) following the manufacturer's protocol, except for using the biotin-labeled primer based on the 3' end adaptor sequences for the priming (5' biotin-ATCTAGAGCGGCCGCAATGGCCACTCTGCGTTGATAC).

Adaptor sequences that would prevent correct assembly of the raw sequencing reads were removed following a similar strategy to that used in NCBI's VecScreen tool, based on the known adaptor sequences used in library preparation.

With the sequencing primers designed based on the two adaptors, the sequence of the 21 bp representing each transcript can be determined via a series of enzymatic reactions on the microbeads.

After digestion by MmeI, the transcripts were ligated with the GEX Adapter 2. With the sequencing primers designed based on the two adaptors, the sequence of the 21 bp representing each transcript can be determined via a series of enzymatic reactions on the microbeads.

Resulting cDNAs were amplified by PCR using antisense (5'-ATTGATGGTGCCTACAG-3') and sense (5'-ATCGTAGGCACCTGAAA-3') primers containing internal BanI restriction sites (underlined), based on the sequences of the 5' adaptor and the RT primer.

In this expanded method denoted as ATLAS (A-dvanced T-elomere L-ength A-nalysis in S. cerevisiae), we have introduced: 1) set of new primers annealing with high specificity to telomeric regions on five different chromosomes; 2) new approach for designing reverse telomere primers that is based on the ligation of an adaptor of a fixed size to telomeric ends.

We also analyzed binding of Crk proteins (Crk and CRKL) and Nck proteins (Nck1 and Nck2), based on the involvement of these adaptors in RTK-dependent actin cytoskeletal rearrangements [41], [42] and our unpublished observations that these SH2 domains bind to distinct targets compared to most RTK effectors.