The problem of determining one or two or more components can be solved with the usual methods based on the absorbance measurement at one or several wavelengths, if the optical response of the solution is free of interferences.
The yield of anthocyanins, quantified following an spectrophotometric method based on the absorbance at 535 nm was 92.92 ± mg/kg of skin.
The proline content per unit FW was calculated based on the absorbance readings at 520 nm using a UV-1200 spectroMacyr (Macy, Shanghai, China).
The contents of NO3 −-N were measured according to the international method (Liang et al. 2012), which was based on the absorbance of NO3 − at 220 nm.
Based on the absorbance curves and the band gap energies evaluated under excitation, ZnS-chitosan bioconjugates were expected to emit light in the UV range (Eg ≥ 3.6 eV).
The adsorption efficiency was examined based on the absorbance at 465 nm [23] using a UV Visible spectrophotometer (Jasco V-360 Spectrophotometer).
Based on the absorbance measurement at OD 570 nm, the concentration of alizarin red S was highest at 2 h in the strain-exposed periosteal-derived stem cells.
The hemolysis percentage of the nanovehicles was quantified based on the absorbance of the supernatant at 541 nm with isotonic PBS and distilled water as control.
Figure 3c summarizes the quantity of SWCNTs eluted by these solutes, which was determined based on the absorbance values at 280 nm; note that the absorbance is insignificantly affected by light absorption of NaSCN itself under these conditions.
This property makes the new method more advantageous over the classical spectrophotometric method based on the absorbance of NHS at 260 nm after reaction with ammonia or hydroxide ion in aqueous solutions [9, 11]. Figure 4 Effects of reaction temperatures on reaction rates of TNBS with ethanolamine.
Specific UV absorption at 254 nm (SUVA254) was calculated based on the absorbance at 254 nm and the DOC concentration [31], and the fluorescence index (FI) was calculated from the ratio of emission intensity at 470 520 nm at excitation 370 nm [24,32].
