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The experimental model was based on that previously reported, with several modifications [ 8- 12].
NBD linked alkyne 26 was prepared by a substitution reaction from NBD-Cl (4-chloro-7-nitrobenzofurazan) 25 by an improved method based on that previously reported in the literature.
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This fixed subset consists of features that we selected based on the previously reported results for which we believe to play a significant role in TIS recognition.
We also constructed an all-in-one CRISPR-Cas9 vector based on the previously reported system20 (Supplementary Fig. 14b).
Cyclic decapeptides were developed based on the previously reported peptide c(LysLeuLysLeuLysPheLysLeuLysGln).
This interaction is expected based on the previously reported interaction of SynGAP1 with Dlg4 [ 46, 47].
Primary adipocytes were isolated based on the previously reported methods with modifications [ 25].
This CO2 flux geothermometer is based on a previously reported CO2 geothermometer that was designed for use with fumarole analysis.
Based on these previously reported findings, we hypothesized that the human kidney is equipped with its own system for metabolizing carnosine.
As an example, the immunoselection procedure followed in this work (since we are handling samples isolated by the AdnaTest) has modified the CTC positivity as based on our previously reported CK-19 assay that is not using any immunoselection [ 6- 8, 10, 32].
The diagnosis of ON was based on previously reported histopathological criteria (Yamamoto et al. 1997, Motomura et al 2004).
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