Sentence examples for based on targeted sequencing from inspiring English sources

However, analysis of ctDNA is often initially based on targeted sequencing, which is both expensive and time consuming.

Since the advent of new sequencing technologies, new approaches have been developed for detecting insertion polymorphism, such as those based on targeted sequencing of TE junctions via sequence capture enrichment procedures [ 15] and in silico methods designed for investigating sequencing data and genome assemblies in repetitive elements [ 16- 18].

To fully explore their potential as mammalian genome manipulation tools for gene therapy and gene discovery, reliable data sets of target sequence preferences based on targeting sequences retrieved form independent integrants are needed for genome-wide target profiling of piggyBac and Tol2 in the human genome.

The universal primer set frame can be applied for multiplex miRNAs detection by simply changing the RT-X part of RT primer frame and RP-Y part of qRT-PCR reverse primer frame based on target sequence.

In this case, the test results were grouped based on target sequence identity.

LSP analysis was performed on the 19 sequenced genomes of mycobacteria in silico, using primers based on target sequence [ 8] (Table  7).

Primers were designed using Primer Express 2.0 (Applied Biosystems), based on target sequences retrieved from the Affymetrix Probe Sequence Database (Liu et al., 2002).

However, based on targeted gene sequencing in Lingulodinium polyedrum [15] and Symbiodinium [16], as well as two genes from Karlodinium veneficum, the patterns described here probably apply to many dinoflagellates.

While this shows that our system has equal sensitivity than Y-STR for males, its principal advantage is that based on targeting autosomal sequences, it enables the analysis of fetal DNA for any sex of the baby.

Drug companies hope to develop new diagnostic tests and, in the longer term, new drugs and treatments based on targeting the specific sequences of DNA involved in disease.

Six pairs of gene-specific primers (five candidate genes and one actin gene) were designed based on target gene sequences using Primer Express software (Additional file 1: Table S1).